Heme Oxygenase-1, a Key Enzyme for the Cytoprotective Actions of Halophenols by Upregulating Nrf2 Expression via Activating Erk1/2 and PI3K/Akt in EA.hy926 Cells

Figure 3

Effects of HP1, HP2, and HP3 on H₂O₂-induced apoptosis in EA.hy926 cells as measured by flow cytometry. (a) Control group; (b) 200 μM H₂O₂ treatment group; (c–f) 1, 5, 10, and 20 μM HP3, respectively, followed by the treatment of 200 μM H₂O₂; (g-h) 10 μM HP2 and HP1, respectively, followed by the treatment of 200 μM H₂O₂. (i) HP3 decreased the percent of apoptotic cells induced by 200 μM H₂O₂ in a dose-dependent manner below 10 μM. (j) HP1, HP2, and HP3 protected EA.hy926 cells against H₂O₂-induced apoptosis. .

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