Heme Oxygenase-1, a Key Enzyme for the Cytoprotective Actions of Halophenols by Upregulating Nrf2 Expression via Activating Erk1/2 and PI3K/Akt in EA.hy926 Cells

Figure 4

Halophenols inhibit H₂O₂-induced production of ROS and TNF-α in EA.hy926 cells. (a) HP1, HP2, and HP3 decreased the ROS release in the order of HP3 > HP2 > HP1 at 10 μM. Confluent cells in 6-well plates were pretreated with 10 μM compound for 6 h, followed by exposure to 200 μM H₂O₂ for 30 min. ROS generation of HP1, HP2, and HP3 was determined by measuring DCFH-DA by flow cytometric analysis. (b) HP1, HP2, and HP3 inhibited the TNF-α secretion in the order of HP3 > HP2 > HP1 at 10 μM. Confluent cells in 24-well plates were pretreated with compound for 6 h, subsequently incubated with 200 μM H₂O₂ for 30 min. TNF-α released into the medium was detected using an ELISA kit according to the manufacturer’s instructions. .

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