Elucidation of Molecular Mechanisms of Streptozotocin-Induced Oxidative Stress, Apoptosis, and Mitochondrial Dysfunction in Rin-5F Pancreatic β-Cells

Figure 5

STZ-induced apoptosis. Apoptosis was measured in Rin-5F cells treated with different doses of STZ at different time intervals by flow cytometry using FACSDiva software. Representative dot plots are shown, and percentage of apoptotic cells is represented as a histogram (a). Activity of caspases was measured in cells (b) treated with different doses of STZ at different time intervals colorimetrically using the respective substrates as described in the vendor’s protocol (R&D Systems Inc.). Results are expressed as mean ± SEM of three experiments. Asterisks indicate significant difference (, ) relative to the untreated control cells.

(a)

(b)