Elucidation of Molecular Mechanisms of Streptozotocin-Induced Oxidative Stress, Apoptosis, and Mitochondrial Dysfunction in Rin-5F Pancreatic β-Cells

Figure 6

Expression of apoptotic protein markers. Total extracts (30 μg protein) from control and Rin-5F cells treated with different doses of STZ at different time intervals were separated on 12% SDS-PAGE and transferred on to nitrocellulose paper by Western blotting. NOS-2 Nrf2, Akt, p-Akt, and GLUT 2 (a) and caspase-3, PARP, Bax, and Bcl-2 proteins (b) were detected using specific antibodies against these proteins. Beta-actin was used as a loading control. The quantitation of proteins bands is expressed as relative ratios normalized against actin or other proteins as appropriate. The figures are representative of three experiments. Asterisks indicate significant difference (, ) relative to the untreated control cells.

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