Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF-κB-Dependent Autophagy

Figure 8

Picroside II reduced the level of SIRT1 and LC3-induced autophagy. (a) The arrows show the autophagic vacuolization in pancreas cells confected with NF-κB or NF-κB shRNA under a phase-contrast microscope (×200). (b) Distribution of GFP-LC3 in pancreas cells confected with NF-κB or NF-κB shRNA under a fluorescent microscope (×200). (c) Western blot analysis of the expression of NF-κB, TNF-α, SIRT1, and LC3. (d) Relative protein level of NF-κB in different groups. (e) Relative protein level of TNF-α in different groups. (f) Relative protein level of SIRT1 in different groups. (g) Relative protein level of LC3 I in different groups. (h) Relative protein level of LC3 II in different groups. NF-κB+, NF-κB overexpression. NF-κB−, NF-κB shRNA. +, picroside II treatment. −, without picroside II treatment. via a picroside II-treated group.

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