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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 7150376, 17 pages
Research Article

Novel Therapeutic Effects of Leonurine On Ischemic Stroke: New Mechanisms of BBB Integrity

1Department of Pharmacology, School of Pharmacy and Institute of Biomedical Science, Fudan University, Shanghai, China
2Department of Pharmacology, School of Pharmacy, Macau University of Science & Technology, Macau

Correspondence should be addressed to Yi-Zhun Zhu; om.ude.tsum@uhzzy

Received 7 January 2017; Revised 10 April 2017; Accepted 16 April 2017; Published 13 June 2017

Academic Editor: Margherita Neri

Copyright © 2017 Qiu-Yan Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1: Real-time RT-PCR primer sequences. Supplementary Figure 1. The expression of HIF-α after OGD/R. The success of the in vitro model was confirmed by the expression of HIF-α. The level of HIF-α was increased at different time after reperfusion. Supplementary Figure 2. HDAC4 regulated the level of NOX4. A: The adenovirus overexpressed HDAC4 in normal condition. B: Overexpression of HDAC4 by adenovirus inhibited the increase of NOX4 induced by OGD/R. C: The specific inhibitor of HDAC4 named Tasquinimod further increased the expression of NOX4 especially at 0.2 μM. Supplementary Figure 3. NOX4 had no effect on the expression of HDAC4. A: The protein level of NOX4 was overexpressed by lentivirus in the normal cells. B: The expression of HDAC4 did not change after over-express of NOX4 under the normal condition. C, D: The inhibitors of NOX4, GKT 137831 and DPI, had no obvious impact on the expression of HDAC4 after OGD/R. Supplementary Figure 4. SCM-198 had no effect on MMP2. In our condition, the mRNA expression of MMP2 after 72h reperfusion had no significant change between control and tMCAO group. At the same time, SCM-198 had no effect on MMP2. Supplementary Figure 5. The expression of NOX2 in vivo. In our tMCAO model, the expression of NOX2 had no change.

  1. Supplementary material