Polydatin Prevents Methylglyoxal-Induced Apoptosis through Reducing Oxidative Stress and Improving Mitochondrial Function in Human Umbilical Vein Endothelial Cells

Figure 3

PD decreases MGO-induced oxidative stress. HUVECs were pretreated with PD for 2 h, followed by stimulation with MGO (200 μM) for 1 h. (a) Cells were stained with DCFH-DA, and the fluorescence intensity was measured at 488/525 nm using a microplate reader. Data shown are mean ± SD of 3 independent experiments and are presented as % of control (first bar). versus Con; ^# versus MGO; ^## versus MGO. (b, c, d) The level of SOD, CAT, and GSH-Px was measured by the respective kits according to the manufacturer’s introductions. Data shown are mean ± SD of 4 independent experiments. versus Con; ^# versus MGO; ^## versus MGO.

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