Therapeutic Potential of Novel Twin Compounds Containing Tetramethylpyrazine and Carnitine Substructures in Experimental Ischemic Stroke

Figure 7

LR134 protected against cell injury in PC12 cells under OGD condition. (a) The quantified data showing cell death determined by flow cytometric analysis. (b) The intracellular Ca²⁺ concentration ([Ca²⁺]i) was measured with a calcium indicator dye, Fluo-3/AM. (c) ATP contents in PC12 cells with different treatments. (d) Mitochondrial membrane potential was detected with the fluorescent probe JC-1 staining and presented by the ratio of JC-1 monomer to J-aggregates followed by flow cytometric analysis. (e) Summarized data showing the relative levels of superoxide (O₂^•−) production in PC12 cells with different treatments. (f) The relative NOX2 mRNA levels in PC12 cells under OGD condition. (g) Representative Western blot gel documents and summarized data showing the relative NOX2 protein levels in PC12 cells with different treatments. versus control-vehicle group, versus OGD-vehicle group ().

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