Figure 2: Hyperoxic preconditioning prevents cisplatin-induced cells death. AD293 (a), Hela (b), and OVCAR-3 (c) cells were treated with nearly pure oxygen (≥90%) preconditioning (2 h) and then cisplatin (50, 35, and 30 μM for AD293, Hela, and OVCAR-3 cells, resp.) was added for an additional 24 h. Cell viability was then determined using the MTT and Neutral Red assays. Data are mean ± SEM; –8 wells for each group; versus both “Air + Veh” and “O2 + Veh” groups (in (a), (b), and (c) parts); , , and versus “Air + CP” group (in (a), (b), and (c) parts).