The Synthetic Lignan Secoisolariciresinol Diglucoside Prevents Asbestos-Induced NLRP3 Inflammasome Activation in Murine Macrophages

Figure 4

Asbestos-induced proinflammatory cytokine secretion is blunted by LGM2605. Release of IL-6 (a), TNFα (c), and HMGB1 (e) was determined at 0, 1, 2, 4, 6, 8, 12, and 24 hours post asbestos exposure. Samples were run undiluted in triplicate, and cytokine concentrations (pg/ml for IL-6 and TNFα and ng/ml for HMGB1) are presented as mean ± SEM. Macrophage mRNA expression of IL-6 (b), TNFα (d), and HMGB1 (f) was determined at 0, 8, and 24 hours post asbestos exposure using qPCR. Levels of target gene mRNA were normalized to β-actin RNA, and values are expressed as mean fold change from CTL at time 0. Data are presented as mean ± SEM. ^∗Statistically significant difference () between ASB- and CTL-treated cells. ^#Statistically significant difference () between ASB- and ASB + LGM2605-treated cells.

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