Oxygen-Sensing Nox4 Generates Genotoxic ROS to Induce Premature Senescence of Nucleus Pulposus Cells through MAPK and NF-κB Pathways

Figure 4

Small interfering RNA against Nox4 (siNox4) alleviated oxidative stress and DNA damage induced by high oxygen tension in NP cells. (a, b) RT-qPCR analysis () and representative immunoblot analysis of Nox4 in NP cells. The knockdown of Nox4 in NP cells was confirmed. (c) ROS production in NP cells (). (d) RT-qPCR analysis of MsrbA, MsrB1, and MsrB2 in NP cells (). (e, f) Immunofluorescence staining of γ-H2A.X and percentage of γ-H2A.X-positive cells in NP cells (). NP cells were transfected with siNox4 or scrambled siRNA control (siCtrl) before high oxygen tension treatment. , value < 0.05, error bars represent standard error.

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