Oxygen-Sensing Nox4 Generates Genotoxic ROS to Induce Premature Senescence of Nucleus Pulposus Cells through MAPK and NF-κB Pathways

Table 1

Primer sequences used in the real-time PCR analysis.

Target gene	Forward primer	Reverse primer
p53	GGGAATCTTCTGGGACGGGACA	CTGGTGGGCAGTGCTCTCTTTG
p21	CTGCCTGGTTCCTTGCCACTTC	GCTCTGGACGGTACGCTTAGGT
p16	CGTCGTGCGGTATTTGCGGTAT	GCGTTGCCAGAAGTGAAGCCA
Rb	AGCAGCCTCAGCCTTCCATACT	TGTTCTGGCTCTGGGTGGTCAG
MsrA	GGCAATGACTGTGGCACGCA	CCTCTCGGATGTCGGTGGTGAT
MsrB1	TCCTGTGGCAAGTGTGGCAATG	TGACTGAGGCTGGAGTGGTTGG
MsrB2	AGCAAGGCAGACTGGCAGAAGA	GGGCTATCACAGCACACGCAAT
Nox4	CGCACAGTCCTGGCTTACCTT	GGCAGCTACATGCACACCTGAG
MMP2	AGTGACGGCTTCCTCTGGTGTT	CAGGGCTGTCCATCTCCATTGC
MMP3	CCTGGCCCGTTTCCATCTCTCT	GGTGCTGACTGCATCGAAGGAC
MMP13	TACGAGCATCCATCCCGAGACC	TGAACCGCAGCACTGAGCCT
ADAMTS4	CCAGGACTTGTGGAGGTGGTGT	AAGAGGTCGGTTCGGTGGTTGT
ADAMTS5	GCTCCTCTTGGTGGCTGACTCT	GCGTTCTTGCTCACCTCCAGAC
IL6	AGCCCACCAGGAACGAAAGTCA	GGAAGGCAGTGGCTGTCAACAA
CCL2	CAGCCCAGAAACCAGCCAACTC	TGAGTAGCAGCAGGTGAGTGGG
CCL5	GACACCACTCCCTGCTGCTTTG	GCACACACTTGGCGGTTCCTT
CXCL10	TGTCGTTCTCTGCCTCGTGCT	CCTGGGTCTCAGCGTCTGTTCA
GAPDH	GTCCATGCCATCACTGCCACTC	GATGACCTTGCCCACAGCCTTG

Msr: methionine sulfoxide reductase; Nox: nicotinamide adenine dinucleotide phosphate oxidase; MMP: matrix metalloproteinase; ADAMTS: a disintegrin and metalloproteinase with thrombospondin motifs; IL: interleukin; CCL: C-C motif ligand; CXCL: C-X-C motif ligand; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.