Research Article

A Carotenoid Extract from a Southern Italian Cultivar of Pumpkin Triggers Nonprotective Autophagy in Malignant Cells

Figure 12

CEN modifies ATP intracellular levels in SAOs cells. Cells were treated for 24 h with NE, CEN (200 μg/ml, w/v), and 2 μM ATRA (positive control). ATP intracellular concentrations were measured as reported in Materials and Methods and expressed as nM ATP. Bar graphs represent the mean ± SD; symbols indicate significance with respect to NE and with respect to untreated cells.