MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress

Figure 3

Effect of MitoTEMPO on oxalate induced intracellular ROS and mtROS generation (a, b). NRK-52E cells were stimulated with oxalate (700 μM) for 3 hours with or without preincubation with MitoTEMPO for 1 hour. (a) The intracellular ROS levels were indicated by the fluorescence intensity of DCF analyzed by flow cytometry. (b) The mtROS levels were indicated by the fluorescence intensity of MitoSOX Red analyzed by flow cytometry. Mean ± SEM of three independent experiments. versus control; versus oxalate treated group (: MitoTEMPO).

(a)

(b)