MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress

Figure 4

Mito ameliorates oxalate induced disruption of mitochondrial membrane potential. NRK-52E cells were stimulated with oxalate (700 μM) for 1 hour with or without preincubation with MitoTEMPO for 1 hour. Mitochondrial membrane potential was observed using a mitochondrial potential-sensitive dye, JC-1. Cells in control group showed bright red fluorescence. Oxalate treatment attenuated red fluorescence compared with control, and pretreatment with MitoTEMPO reversed these changes (: MitoTEMPO).