MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress

Figure 6

MitoTEMPO selectively modulated the protein expression of NADPH oxidase subunits in NRK-52E cells exposed to oxalate. NRK-52E cells were stimulated with oxalate (700 μM) for 24 hours with or without preincubation with MitoTEMPO for 1 hour. Oxalate treatment decreased the protein expression of Nox4 and elevated the protein expression of p22 significantly compared with the control group. MitoTEMPO pretreatment elevated the protein expression of Nox4 and decreased the protein expression of p22. The protein expression of Nox2 was not changed after oxalate stimulation with or without MitoTEMPO pretreatment. versus control; versus oxalate treated group (: MitoTEMPO).