Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation

Figure 3

ASP treatment reduces proinflammatory cytokines levels and NF-κB activation. (a) Serum IL-6 and serum (b) TNF-α levels were measured by ELISA. Data are reported as means ± SEM ( per group). ^#, ^## versus control. ^#, ^## versus control. , versus ACD. (c) Immunoblots of protein expressions of IKKα, NF-κB p65, and p-IκBα in the livers of ACD rats. GAPDH served as the loading control. One representative blot of 3 independent experiments is shown. (d) The hepcidin mRNA levels were quantified by qRT-PCR. Values shown were the means of fold changes in Hamp gene expression relative to β-actin (2^−ΔΔCt) ± SEM, . (e) ASP significantly reduced IL-6 secretion evoked by LPS (). Values are means ± SEM from 3 independent experiments. (f, g) ASP suppresses LPS-induced NF-κB activation and hepcidin expression in HepG2 cells. Immunoblots of protein expressions of hepcidin, NF-κB, p-IκBα, STAT3, and p-STAT3. β-Actin and histone 3.1 served as the loading control. ^# and ^## compared with the control group; and compared with the LPS group.

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