RTA-408 Protects Kidney from Ischemia-Reperfusion Injury in Mice via Activating Nrf2 and Downstream GSH Biosynthesis Gene

Figure 2

Morphological improvement of RTA-408 on renal ischemia-reperfusion injury. (a) PAS-stained histologic photomicrographs in the indicated area from the sham, vehicle + I/R, and 408 + I/R groups at 24 h reperfusion (). G indicated glomerulus; black arrows indicated brush border; red arrowheads indicated necrotic tubules; and black arrowheads indicated injured tubules. (Magnification 400x; scale bar 50 μm). (b) Quantification of ATN score in the cortex and outer medulla of the above three groups. Data were presented as mean ± SD. versus the vehicle + I/R group. (c) Immunohistochemistry was performed to detect MPO-positive cells in kidneys of the above three groups. Red arrows indicate MPO-positive cells. (Magnification 400x; scale bar 50 μm). (d) Quantification of MPO-positive cells per high-power field in different groups. Values are expressed as mean ± SD. versus the vehicle + IR group.

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