Mulberry Anthocyanin Extract Ameliorates Oxidative Damage in HepG2 Cells and Prolongs the Lifespan of Caenorhabditis elegans through MAPK and Nrf2 Pathways

Figure 1

Identification and quantification of anthocyanins in MAE by HPLC analysis. The mobile phase A was 8.5% formic acid aqueous solution and the mobile phase B was formic acid/acetonitrile/methanol/water (8.5 : 22.5 : 22.5 : 41.5, v/v/v/v). A linear gradient program: 0–35 min from 7 to 25% B, 35–45 min from 25 to 65% B, 45-46 min from 65 to 100% B, and 46–50 min 100% B. The flow rate was 1.0 mL/min and the absorption spectrum was recorded at 535 nm. The column was operated at a temperature of 30°C. Three replicates were performed in the analysis.