Mulberry Anthocyanin Extract Ameliorates Oxidative Damage in HepG2 Cells and Prolongs the Lifespan of Caenorhabditis elegans through MAPK and Nrf2 Pathways

Figure 3

MAE reduced H₂O₂-induced intracellular ROS and O₂⁻ levels in HepG2 cells. Cells with different treatments were incubated with H₂DCFDA or DHE for (a) ROS and (b) O₂⁻ analysis. The results are shown as representative microscopic scans: (A) control, (B) cells induced by H₂O₂ (300 μM, 4 h), (C) GSH (1 mM), and (D) MAE (100 μg/mL). The quantitative analysis of fluorescence from three independent replicates was used by the Image-Pro Plus 6.0 software. Values with different letters above are significantly different, , one-way ANOVA test.

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