Mulberry Anthocyanin Extract Ameliorates Oxidative Damage in HepG2 Cells and Prolongs the Lifespan of Caenorhabditis elegans through MAPK and Nrf2 Pathways

Figure 7

Effective assessment of MAE on oxidative protection in different mutants. (a) Lifespans of daf-16 and pmk-1. (b) Lipofuscin levels of daf-16 on the 10th day of adulthood. (c) PMK-1 expression in mutant AY102 worms. (d) SKN-1 activity was imaged for GFP expression indicative of SKN-1 activation of the gst-4p::gfp reporter in mutant SPC167 worms. (A) control, (B) MAE, (C) paraquat (1 mM), and (D) paraquat in the presence of MAE. (e) The quantitative analysis of fluorescence. Densitometry analysis was performed using the Image-Pro Plus 6.0 software. Values with different letters above are significantly different, , one-way ANOVA test.

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