Inhibition of miR-302 Suppresses Hypoxia-Reoxygenation-Induced H9c2 Cardiomyocyte Death by Regulating Mcl-1 Expression

Figure 1

miR-302 regulates the transcriptional activity of Mcl-1 by targeting its 3′UTR. (a) The 3′UTR sequence (2209–2232) of Mcl-1 contains a miR-302a-binding site as predicted by miRanda (http://microRNA.org). (b) Mcl-1 is a miR-302 target based on dual-luciferase reporter results. Luciferase activity was evaluated in H9c2 cells transfected with the PsiCheck2 vector only or with PsiCheck2 vectors containing the Mcl-1 3′UTR with nonsense control (NC) or a miR-302 mimic (m302). Data are presented as the mean ± STD of six experiments. .

(a) Sequence matching between 3′UTR of Mcl-1 gene and miR-302 binding site

(b) Dual-luciferase reporter assay