The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

Figure 1

Transient overexpression of the recombinant plasmid DNAJB6(S) and small interfering RNAs in LN18 glioblastoma cells. LN18 cells were transiently transfected to overexpress (Ov) pMOCK or DNAJB6(S) alone or were cotransfected with each of the vectors together with negative control siRNA (NC) or three different DNAJB6(S) siRNAs (siRNA 1, siRNA 2, or siRNA 3). Cells were analyzed to determine mRNA levels (a) and immunoblotted with an anti-DNAJB6 antibody or c-myc epitope-tagged antibody (b). Data are expressed relative to pMOCK cells and presented as means ± SEM (, ). GAPDH and β-actin levels are shown as internal controls.

(a)

(b)