The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

Figure 2

DNAJB6(S) transient overexpression protects against MPP⁺-induced apoptosis. (a) LN18 cells transiently overexpressing a control plasmid (pMOCK) or pDNAJB6(S) were exposed to 500 µM MPP⁺ for 48 h, and apoptosis was analyzed using flow cytometry. The total numbers of early (Annexin V⁺/PI⁻) and late (Annexin V⁺/PI⁺) apoptotic cells are expressed as percentages. (, mean ± SEM, and compared with control at 48 h, and compared with Ov-pMOCK.) (b) Cell morphological changes and patterns were visualized using a phase-contrast microscope. Bar is 100 µm.