The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

Figure 3

Establishment of DNAJB6(S) stable overexpressing LN18 cells. (a) The protein levels of DNAJB6(S) (left) and the protein levels of recombinant DNAJB6(S) with a C-terminal c-myc epitope (right) in LN18 cell lines stably transfected by pMOCK or pDNAJB6(S) were determined by western blotting using an anti-DNAJB6 or anti-c-myc antibody. (b) The expression levels of DNAJB6(S) in pMOCK cells and pDNAJB6(S)-2 cells were determined by western blot analysis and RT-PCR. (c) Subcellular localization of DNAJB6(S). Staining with anti-mouse IgG was performed as a negative control (left), and FITC secondary antibody was used to detect specific immunostaining; nuclei were stained with DAPI (middle panel). Bar is 10 µm, 1000x magnification.

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