The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

Figure 4

DNAJB6(S) stable overexpression in LN18 cells protects against apoptosis. (a) LN18 cells stably transfected by pMOCK or pDNAJB6(S) were exposed to either vehicle (Ctrl) or 500 µM MPP⁺ for 48 h, and apoptosis was assessed using flow cytometry. The total numbers of early (Annexin V⁺/PI⁻) and late (Annexin V⁺/PI⁺) apoptotic cells are expressed as percentages. (b)–(g) Cell morphological changes and patterns were visualized. Bar is 100 µm. (h) Protein levels of DNAJB6(S) were evaluated by western blot assay after treatment with 500 µM MPP⁺ for 48 h. β-Actin was used as an internal control. (, mean ± SEM, and compared with control-treated pMOCK or pDNAJB6 and compared with MPP⁺-treated pMOCK).