The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

Figure 5

DNAJB6(S) attenuates the formation of ROS in MPP⁺-treated cells. LN18 cells stably transfected by pMOCK and pDNAJB6(S) were exposed to either vehicle (Ctrl) or 500 µM MPP⁺ for 48 h, and intracellular ROS levels were measured with a CM-H2DCFDA probe using flow cytometry. The data were analyzed using Student’s -test. (, mean ± SEM, and compared with pMOCK.)