The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

Figure 6

DNAJB6(S) prevents the loss of mitochondrial membrane potential () in MPP⁺-treated cells. (a) LN18 cells stably transfected by pMOCK and pDNAJB6(S) were exposed to either vehicle (Ctrl) or 500 µM MPP⁺ for 48 h, and the loss in was measured by flow cytometry using the JC-1 mitochondrial probe. The values were calculated as the ratio of red/green signal and are expressed as percentage of the control. (, mean ± SEM, and compared with control-treated pMOCK or pDNAJB6 and compared with MPP⁺-treated pMOCK). (b)–(e) Cells treated with the conditions described in (a) were fixed and stained with the mitochondrial marker MitoTracker Red CMXRos and visualized by fluorescence microscopy. Bar is 50 µm.