The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

Figure 7

DNAJB6(S) inhibits p18 Bax cleavage and caspase-9 activation in MPP⁺-treated cells. LN18 cells stably transfected by pMOCK or pDNAJB6(S) were treated with or without 500 µM MPP⁺ for 48 h. The cells were harvested, and mitochondrial and cytosolic fractions (F) were prepared. The expression levels of p18 Bax and caspase-9 cleavage were analyzed by western blotting. An anti-Bax antibody detected both full-length Bax (20 kDa) and p18 Bax. β-Actin was used as a loading control and COX-II was used for mitochondrial marker.