The Neuroprotective Effects of Brazilian Green Propolis on Neurodegenerative Damage in Human Neuronal SH-SY5Y Cells

Figure 4

The effect of methanol extracts of propolis on the IL-1β-induced impairment of BDNF-induced Arc expression in SH-SY5Y cells. (a) SH-SY5Y cells were treated with the indicated concentrations of IL-1β for 48 h. The cell viability of SH-SY5Y was then measured using a CCK-8 Assay Kit. (b) SH-SY5Y cells were pretreated with IL-1β (20 ng/mL) for 6 h followed by incubation with BDNF (10 ng/mL) for 2 h. Propolis was treated 2 h before BDNF application. (c) The expression of Arc after treatment with IL-1β, propolis, or BDNF. (d) The quantitative analysis of Arc protein expression. The results are expressed as the mean ± SEM ( each), and the asterisks indicate a statistically significant difference from the indicated group value (, ).

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