Oxidative Stress Regulation on Endothelial Cells by Hydrophilic Astaxanthin Complex: Chemical, Biological, and Molecular Antioxidant Activity Evaluation

Table 1

The sequences of primers used for study of profile of RNAs using q-PCR.

Target genes	Primer	Primer sequence (5-3)	Annealing temperature (°C)
PTEN	Forward	TCCCAGTCAGAGGCGCTATG	60
	Reverse	CACAAACTGAGGATTGCAAGTTC
Nrf2	Forward	GAGACAGGTGAATTTCTCCCAAT	59
	Reverse	TTTGGGAATGTGGGCAAC
HO1	Forward	ACGGCTTCAAGCTGGTGATG	61
	Reverse	TGCAGCTCTTCTGGGAAGTAG
NQOI	Forward	ATGTATGACAAAGGACCCTTCC	62
	Reverse	TCCCTTGCAGAGAGTACATGG
Bax	Forward	ATCCAGGATCGAGCAGGGCG	64
	Reverse	GGTTCTGATCAGTTCCGGCA
AKT1	Forward	GTTTGCCGGAATCAATTTTC	60
	Reverse	AGCCAGAGCTGTGATCTCCTT
GAPDH	Forward	GAGCCAAAAG GGTCATCATC	63
	Reverse	TAAGCAGTTGGTGGTGCAGG
Caspase-3	Forward	TGTGAGGCGGTTGTGGAAGAGT	63
	Reverse	AATGGGGGAAGAGGCAGGTGCA
eNOS	Forward	ATCTCCGCCTCGCTCATG	61
	Reverse	GAGCCATACAGGATTGTCGC