Modulatory Effect of Fermented Papaya Extracts on Mammary Gland Hyperplasia Induced by Estrogen and Progestin in Female Rats
Effect of SPE supplementation on oxidative stress in mammary gland hyperplasia of rats induced by estradiol benzoate and progestin administration. (a–f) Representative images of 8-oxo-dG immunostaining in the mammary glands (200x). No apparent 8-oxo-dG-positive cells were observed in the control group (a) and the FPE-treatment-alone group (b). A high percentage of 8-oxo-dG-positive cells were observed in the estradiol benzoate and progestin treatment group (c). Scale bar, 100 μm. However, the percentage of 8-oxo-dG-positive cells decreased upon treatment with FPEs at 30 ml/kg (d), 15 ml/kg (e) and 5 ml/kg (f). (g) Quantification of 8-oxo-dG-positive cells in the mammary glands by ELISA. Data are presented as mean ± SEM. A compared with the control group; B compared with the EB + PR group.