Hydrogen-Rich Water Ameliorates Total Body Irradiation-Induced Hematopoietic Stem Cell Injury by Reducing Hydroxyl Radical

Figure 3

HW attenuates the TBI-induced decrease in BMCs, hematopoietic stem cells, and progenitor cells. (a) The bar graph shows the number of BMCs per femur. (b) The bar graph shows the percentage of c-kit⁺ cells among lineage-negative cells. (c) The bar graph shows the percentage of HPCs (Lineage^-sca1^-c-kit⁺BMCs) among lineage-negative cells. (d) The bar graph shows the percentage of LSKs (Lineage^-sca1⁺c-kit⁺BMCs) among lineage-negative cells. (e) The bar graph shows the percentage of CD34^-LSKs among LSK cells. (f) The bar graph shows the percentage of CD34⁺LSKs among LSK cells. The data are presented as the mean ± SEM of cell numbers per femur (). (g) Representative FACS analysis of the percentage of LSKs and HPCs. (h) Representative analysis of the percentage of CD34^-LSKs and CD34⁺LSKs. All the data represent the mean ± SEM (); versus 0 Gy control; versus 4 Gy control.

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