Hydrogen-Rich Water Ameliorates Total Body Irradiation-Induced Hematopoietic Stem Cell Injury by Reducing Hydroxyl Radical

Figure 6

HW increases proliferation and decreases apoptosis in irradiated c-kit⁺ cells. (a) The bar graph shows the percentage of G0 phase (ki67^-PI^-) cells among BMCs. (b) The bar graph shows the percentage of G1 phase (ki67⁺PI^-) cells among BMCs. (c) The bar graph shows the percentage of S/G2/M phase (ki67⁺PI⁺) cells among BMCs. (d) The bar graph shows the percentage of early apoptotic cells (annexin V⁺PI^-). (e) The bar graph shows the percentage of late apoptotic cells (annexin V⁺PI⁺). (f) Representative FACS analysis of cell cycle status in BMCs. (g) Representative FACS plots of apoptosis in c-kit⁺ cells. All data represent the mean ± SEM (). versus 0 Gy control; versus 4 Gy control.

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