Hydrogen-Rich Water Ameliorates Total Body Irradiation-Induced Hematopoietic Stem Cell Injury by Reducing Hydroxyl Radical

Figure 7

HW decreases DNA damage and oxidative DNA damage in irradiated c-kit⁺ cells. (a) The bar graph shows MFI of γ-H2AX in c-kit⁺ cells, as detected by flow cytometry. (b) Representative FACS plots of γ-H2AX. (c) Representative images of γ-H2AX in c-kit⁺ cells, as detected by immunofluorescence (IF). (d) The bar graph shows the percentage of 8-oxoG positive cells in c-kit⁺ cells. (e) Representative images of 8-oxoG in c-kit⁺ cells, as detected by IF. All the data represent the mean ± SEM ( in panel (a) and in panel (d)); versus 0 Gy control; versus 4 Gy control.

(a)

(b)

(c)

(d)

(e)