Modulation of Glutathione Hemostasis by Inhibition of 12/15-Lipoxygenase Prevents ROS-Mediated Cell Death after Hepatic Ischemia and Reperfusion

Figure 4

Quantitative serum analyses of caval blood gained after inducing hepatic IRI or, respectively, after sham operation. (a) Quantification of the increase of liver enzymes in sham-operated mice after DMSO and Baicalein administration by calculating the toxic factor. The toxic factor is calculated as a ratio of enzyme elevation after drug administration compared to the control group over the control group itself. The data show that Baicalein and DMSO administration relevantly increase AST, ALT, and GLDH levels. (b) Assessment of attenuation of the liver enzyme elevation post DMSO and Baicalein pretreatment after inducing hepatic IRI by calculating a relative increase in enzyme activation. This is generated as ratio of the calculated toxic factor after IRI over the previously calculated toxic factor of the respective sham-operated mice. Therefore, the relative enzyme increase for the sham group matches 100% for each enzyme and pretreatment to be investigated. Hence, it can be stated that Baicalein pretreatment after IRI leads to an acknowledgeable decrease of liver enzyme elevation compared to the respective sham-operated mice, significant for AST activation (−50.3%; ) and GLDH activation (−65.8%; ).

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