Modulation of Glutathione Hemostasis by Inhibition of 12/15-Lipoxygenase Prevents ROS-Mediated Cell Death after Hepatic Ischemia and Reperfusion

Figure 5

Quantification of spectral photometric analyses of intracellular activity of glutathione oxidation using Tietze’s kinetic test. Activity was calculated as a ratio of measured amount of oxidized glutathione disulfide (GSSG), the product of GPX4, over its substrate glutathione (GSH). Pretreatment with Baicalein showed a significant increase in glutathione oxidation by +54.5% (), DMSO solely in a more discrete fashion (+16.7%).