Figure 3: Myricetin inhibited the nuclear translocation of p-P65 and secretion of inflammatory cytokines. (a) Frozen section for the detection of p-P65 by immunofluorescence, white arrows showed the merging of p-P65 and nuclei (). (b) The calculation of p-P65-positive nuclei among different groups (). (c), (d), and (e) The relative mRNA expression of interleukin 6 (IL-6) and IL-1β and tumor necrosis factor (TNF-α) (). GAPDH was selected as the internal reference, all of the mRNA was normalized to GAPDH before relative quantitative statistics. Data were presented as means ± SEM. versus CON or M and # versus STZ.