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Figure 9: Myricetin blocked nuclei translocation of p65 and apoptosis in NRCM. (a) After 36 h of high glucose treatment, p65 were phosphorylated and translocated into the nuclei. (b) After 72 h of high glucose treatment, apoptosis-associated proteins (Bax and c-caspase 3) were accumulated in NRCM, which could be blocked by treatment of myricetin. (c), (d), (e), and (f) Histogram showed the fold change of p-P65, Nuc-p65, Bax, and c-caspase 3; all of the proteins were normalized to GAPDH or histone 3 before relative quantitative statistics, and all experiments were repeated 3 times independently. Data were presented as means ± SD. as compared with CON or M group and # as compared with HG group.