Research Article

Resveratrol-Enriched Rice Attenuates UVB-ROS-Induced Skin Aging via Downregulation of Inflammatory Cascades

Figure 3

UVB-MMP1-mediated protein expression and the secretion of MMP-1 and PIP were measured using Western blot analysis and an ELISA kit, respectively. NHDF cells were incubated for 72 h with or without UVB exposure and treated with or without NR, RR, and R. (a) MMP-1 and PIP expression, accompanied by the corresponding (b, c) densitometric analysis results (d, e) and the quantities MMP-1 and PIP secreted into the treated medium supernatant. (f) TGF-β and elastin expression, accompanied by the corresponding (g, h) densitometric analysis results, taking the UVB-treated control as 100%. All data are presented as the mean ± SEM of three independent experiments. #, ##, ### versus the NC and , versus the UVB control. NC is normal control, Ctl is UVB control, NR is normal rice, RR is resveratrol-enriched rice, and R is resveratrol. $$$p < 0.001 versus UVB control represents the inhibition of elastin expression by resveratrol treatment. NR and RR were treated in μg/mL, and R was treated in μM.