Resveratrol-Enriched Rice Attenuates UVB-ROS-Induced Skin Aging via Downregulation of Inflammatory Cascades

Figure 6

Proinflammatory cytokines (TNF-α and IL-6) secretion and inflammatory markers (iNOS and COX-2) expression. NHDF cells were treated with or without UVB (144 mJ/cm²) and with NR, RR, and R for 72 h. Proinflammatory cytokines were measured in cell supernatant using an ELISA kit, and protein expression of iNOS and COX-2 was measured using Western blot analysis. (a, b) TNF-α secretion in NHDF cells without UVB-conditioned medium and with UVB-conditioned medium. (c, d) IL-6 secretion in NHDF cells without UVB-conditioned medium and with UVB-conditioned medium. (e) iNOS and COX-2 protein expression from multiple experiments. (f, g) Bar graphs represent quantitative densitometric results of upper bands. All data are presented as the mean ± SEM of three independent experiments. ^### versus the NC and , , versus the UVB-irradiated control. NC is normal control, Ctl is UVB control, NR is normal rice, RR is resveratrol-enriched rice, and R is resveratrol. NR and RR were treated in μg/mL, and R was treated in μM.

(a)

(b)

(c)

(d)

(e)

(f)

(g)