The Effects of Blast Exposure on Protein Deimination in the Brain

Figure 2

Blast-induced deimination of proteins in porcine brain. Brain samples were collected 2 weeks following a single blast exposure (average pressure = 46 psi). Homogenates of control (C) and the blast-exposed (B) cerebral cortex were prefractionated by LP-IEF. The resulting pH fractions were further fractionated by 1-dimensional SDS-PAGE (a) and analyzed for protein deimination by western blotting (b) using the mouse monoclonal 6B3 anti-protein citrulline antibody. Immunoreactive features affected by TBI (numbered, panel (b)) were mapped to corresponding bands in a Coomassie-stained protein gel (numbered, panel (a)). These were collected, identified, and mapped for site-specific deimination by peptide mass fingerprinting using liquid chromatography and tandem mass spectrometry (LC MS/MS).

(a)

(b)