Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis

Figure 3

Three-dimensional images of J774.1 macrophages after AGE uptake. The J774.1 macrophages were incubated with 20 μg/ml of AGE-2 (a) or AGE-3 (b) at 37°C for 2 hrs. The immunoreactivities in the cells (green) were observed under confocal microscopy with DAPI staining (blue). Serial sections in the z-axis are shown in the right-hand panels. Arrowheads in (c) indicate AGE-3-positive signals in the nuclear compartment.

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(b)

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