Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis

Figure 5

AGE-2 or AGE-3 stimulated ROS production in J774.1 macrophages. The J774.1 macrophages were incubated with 100 μg/ml of AGE-2, AGE-3 or BSA at 37°C for 24 hrs. ROS production was detected by DCF fluorescence after the hydrolysis of DCF-AM inside the cells. Representative images from three separate experiments are shown.