Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis

Figure 8

Double immunostaining of NF-κB p65 and 4-hydroxynonenal after stimulation with AGE-2 and AGE-3 in J774.1 macrophages. The J774.1 macrophages were incubated with 100 μg/ml of AGE-2 or AGE-3 at 37°C for 12 hrs. (a) The cells were stained with anti-NF-κB p65 and anti-4-hydroxynonenal together with DAPI. White arrowheads show the relative expression of NF-κB p65 in the nucleus after incubation with AGE-2 and AGE-3. (b) After incubation, the J774.1 macrophages were collected, and nuclear and cytosolic fractions were prepared for SDS-PAGE and NF-κB p65 detection by Western blotting. Representative results from three independent experiments are shown.

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(b)