Research Article

Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells

Figure 5

MCA-hMSCs rescued injured cells by reducing oxidative stress. (a) Healthy hMSCs with or without MCA treatment were labeled with CFDA before coculture. After 24 h coculture with H2O2-treated hMSCs, all cells were harvested, stained with and MitoSOX Red, and subjected to flow cytometric analysis. The florescence of MitoSOX Red was determined separately in the CFDA-labeled and CFDA-unlabeled cells. The proportion of injured cells (CFDA-unlabeled) with MitoSOX Red in coculture containing MCA-treated hMSCs was 24.4% as compared to the 37.1% in the control hMSCs coculture (b). However, the proportion of MitoSOX Red cells in the CFDA-labeled healthy cells did not differ much (c). The viability of injured cells increased after coculture (d). , as compared with the control.
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