Mechanism of Mechanical Trauma-Induced Extracellular Matrix Remodeling of Fibroblasts in Association with Nrf2/ARE Signaling Suppression Mediating TGF-β1/Smad3 Signaling Inhibition

Figure 8

The relationship of Nrf2 signaling and TGF-β1 signaling in mechanical injury-induced ECM remodeling. (a) The effect of Nrf2 overexpression and LY2109761 on mechanical strain-induced TGF-β1 signaling inhibition. (b) The effect of Nrf2 knockdown and TGF-β1 on mechanical strain-induced TGF-β1 signaling inhibition. (c) The effect of Nrf2 overexpression on mechanical strain-induced nuclear translocation inhibition of Smad2/3. Original magnification: ×100. ; every experiment was repeated for 3 times. CON: control group; CMS: cyclic mechanical strain; shNfe2l2: Lv-shNfe2l2 transfection established Nrf2 silencing L929 cells; LV-Nfe2l2: Lv-Nfe2l2 transfection established Nrf2 overexpressing L929 cells; TGF-β1: mTGF-β1 pretreatment; LY2109761: TGF-β1 signaling inhibitor LY2109761 pretreatment; CON-CMS: 5333 μstrain mechanical strain-treated normal L929 cells; LV-Nfe2l2-CMS: 5333 μstrain mechanical strain-treated Nrf2 overexpressing L929 cells.

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