Mechanism of Mechanical Trauma-Induced Extracellular Matrix Remodeling of Fibroblasts in Association with Nrf2/ARE Signaling Suppression Mediating TGF-β1/Smad3 Signaling Inhibition

Table 1

The primers for the quantitative real-time polymerase chain reaction.

Gene name	Gene ID	Primer sequence (5-3)	Amplicon size (bp)
Collagen I (A1)	NM_007742.3	F: AAGAAGCACGTCTGGTTTGGAG	175
		R: GGTCCATGTAGGCTACGCTGTT
Collagen III (A1)	NM_009930	F: GTGGCAATGTAAAGAAGTCTCTGAAG	191
		R: GGGTGCGATATCTATGATGGGTAG
Elastin	NM_007925.4	F: CTGGTGTTGGTCTTCCAGGT	112
		R: GCTTTGACTCCTGTGCCAGT
Nrf2	NM_010902.3	F: CTGGCTGATACTACCGCTGTTC	208
		R: AGGTGGGATTTGAGTCTAAGGAG
GPx1	NM_001329527.1	F: CAATGTCGTTGCGGCACACC	135
		R: CCTCAAGTACGTCCGACCTG
MnSOD	NM_013671.3	F: AACTCAGGTCGCTCTTCAGC	121
		R: CTCCAGCAACTCTCCTTTGG
TGF-β1	NM_011577	F: CTAATGGTGGACCGCAACAAC	99
		R: CACTGCTTCCCGAATGTCTGA
Smad2	NM_001252481.1	F: GCTCAAGGCAATCGAAAACT	121
		R:CCGAGGCACTAAGACTGGAG
Smad3	NM_016769.4	F: CACAGCCACCATGAATTACG	120
		R:TGGAGGTAGAACTGGCGTCT
MMP-2	NM_008610.3	F: ACCCAGATGTGGCCAACTAC	121
		R: AAAGCATCATCCACGGTTTC
MMP-9	NM_013599.4	F: AGACGACATAGACGGCATCC	116
		R: TGGGACACATAGTGGGAGGT
TIMP-1	NM_001044384.1	F: CCCAGAAATCAACGAGACCACC	241
		R: ACGCCAGGGAACCAAGAAGC
TIMP-2	NM_011594.3	F: GACACGCTTAGCATCACCCA	127
		R: CCATCCAGAGGCACTCATCC
GAPDH	NM_008084.2	F: AGGAGCGAGACCCCACTAACA	247
		R: AGGGGGGCTAAGCAGTTGGT