Cardioprotective Effects of Transfusion of Late-Phase Preconditioned Plasma May Be Induced by Activating the Reperfusion Injury Salvage Kinase Pathway but Not the Survivor Activating Factor Enhancement Pathway in Rats

Figure 1

Experimental protocols. The experiment was divided into two parts. Part 1 was designed to ascertain the impact of preconditioned plasma on survival kinase phosphorylation in normal myocardium that was not subjected to myocardial ischemia reperfusion, and part 2 was designed to ascertain the impact in ischemic myocardium, including the central area of the area at risk and the border area of the area at risk, which was obtained after myocardial ischemia reperfusion. After samples of myocardium were collected, Western blots were performed on these samples to detect the phosphorylation of survival kinases.