Cardioprotective Effects of Transfusion of Late-Phase Preconditioned Plasma May Be Induced by Activating the Reperfusion Injury Salvage Kinase Pathway but Not the Survivor Activating Factor Enhancement Pathway in Rats

Figure 3

Phosphorylation of STAT3, Akt, and Erk1/2 in ischemic myocardium obtained from the central area of the area at risk. The central area of the area at risk was collected at 15 minutes after myocardial reperfusion. Equal amounts of myocardial protein lysate from the central area of the area at risk of rats receiving nonpreconditioned plasma transfusion (group NPP-IR), normal saline (group NS-IR), or preconditioned plasma (group PP-IR) were subjected to Western blot ( per group). Error bars represent the means ± SD. (a) Representative images of Western blots. Phosphorylated STAT3 (p-STAT3) and STAT3, phosphorylated Akt (p-Akt) and Akt, phosphorylated Erk1/2 (p-Erk1/2) and Erk1/2, and GAPDH are shown. GAPDH was used as a loading control. (b) Phosphorylation level of STAT3, expressed as the ratio of p-STAT3 to STAT3. (c) Phosphorylation level of Akt, expressed as the ratio of p-Akt to Akt. (d) Phosphorylation level of Erk1/2, expressed as the ratio of p-Erk1/2 to Erk1/2. versus group NPP-IR; ^# versus group NS-IR.

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